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Optimization of the LFIA assay with pipette-based spot deposition of antibodies, PEG-Er UCNPs as a label, and HSA as an analyte. Optimization of a nitrocellulose membrane, b coating buffer, c coating time, d antibody coating concentration, e blocking buffer, f blocking technique, g type and dilution of UCNP-based label, and h preincubation time of the dip-stick method. The concentration of HSA was 10 ng/mL (panels a , c , e , f , g , and h ) or 100 ng/mL (panels b and d ). Error bars represent standard deviations, and hatched columns indicate the optimal conditions

Journal: Mikrochimica Acta

Article Title: Label matters: comparing gold nanoparticles and nanoshells with upconversion nanoparticles for quantitative lateral flow immunoassays

doi: 10.1007/s00604-026-08015-5

Figure Lengend Snippet: Optimization of the LFIA assay with pipette-based spot deposition of antibodies, PEG-Er UCNPs as a label, and HSA as an analyte. Optimization of a nitrocellulose membrane, b coating buffer, c coating time, d antibody coating concentration, e blocking buffer, f blocking technique, g type and dilution of UCNP-based label, and h preincubation time of the dip-stick method. The concentration of HSA was 10 ng/mL (panels a , c , e , f , g , and h ) or 100 ng/mL (panels b and d ). Error bars represent standard deviations, and hatched columns indicate the optimal conditions

Article Snippet: For dip-stick tests with piezo-driven line deposition (Fig. c), antibodies were dispensed onto the test and control lines on the nitrocellulose membrane using the sciFLEXARRAYER S3 (Scienion, Germany), and the membrane was then attached to a plastic support along with an absorbent and a sample pad.

Techniques: Transferring, Membrane, Concentration Assay, Blocking Assay